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    Vector Laboratories instruction
    Instruction, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 441 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/instruction/product/Vector Laboratories
    Average 95 stars, based on 441 article reviews
    instruction - by Bioz Stars, 2026-02
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    Schematic overview of the workflow for cytoplasmic and nuclear protein fractionation and Immunoblotting The diagram outlines the key steps involved in the subcellular fractionation of cytoplasmic and nuclear proteins from cultured mammalian cells. The workflow includes cell harvesting, gentle lysis to extract the cytoplasmic fraction, washing of intact nuclei to eliminate cytoplasmic contamination, and subsequent nuclear lysis for nuclear protein isolation. Protein fractions are quantified using the BCA assay, followed by SDS-PAGE and Immunoblotting to assess the subcellular localization of target proteins. Figure created with BioRender.com .

    Journal: STAR Protocols

    Article Title: Protocol for nucleo-cytoplasmic fractionation of mammalian cells to study protein translocation via immunoblotting

    doi: 10.1016/j.xpro.2025.104265

    Figure Lengend Snippet: Schematic overview of the workflow for cytoplasmic and nuclear protein fractionation and Immunoblotting The diagram outlines the key steps involved in the subcellular fractionation of cytoplasmic and nuclear proteins from cultured mammalian cells. The workflow includes cell harvesting, gentle lysis to extract the cytoplasmic fraction, washing of intact nuclei to eliminate cytoplasmic contamination, and subsequent nuclear lysis for nuclear protein isolation. Protein fractions are quantified using the BCA assay, followed by SDS-PAGE and Immunoblotting to assess the subcellular localization of target proteins. Figure created with BioRender.com .

    Article Snippet: This can be achieved by mixing 40 μl of Reagent B with 1,960 μL of Reagents A (following the Thermo Scientific BCA protein Assay Kit instructions, Cat. No. 23225).

    Techniques: Fractionation, Western Blot, Cell Culture, Cell Harvesting, Gentle, Lysis, Isolation, BIA-KA, SDS Page